minsage
minsage (mininmal sample size for genotypes) is designed to calculate the sample size of genotypes minimally required to ensure that all alleles with a specified frequency at one locus are detected with a given confidence.
The program is based on the following publication:
Gregorius, H.-G. (1980) The probability of losing an allele when diploid genotypes are sampled. Biometrics, 36, 643-652.
minsage is started by typing "minsage".
Within the program, you are prompted to specify the following parameters:
- allele frequency: minimum allele frequency a that is to be detected
- confidence: confidence for detecting the allele
- uniformly distributed alleles or biallelic markers: The allele can be set to be the less frequent allele of a biallelic marker. Otherwise, if neither the number of alleles nor the genotypic frequencies are known, alleles can set to be uniformly distributed.
The output renders the minimal sample size N of genotypes needed to detect alleles of frequency a with the specified confidence. The results are given both for the case that Hardy- Weinberg equilibrium can be or cannot be assumed.
Copyright: Andreas Ziegler
Contact: Inke.Koenig@imbs.uni-luebeck.de